Figure 4.

Expression of candidate proteins in glomeruli. (a) Protein lysates from isolated glomeruli (n=3 kidneys from each group) were used to immunoblot for CYP4A12A and ACY3. Levels of CYP4A12A and ACY3 appeared markedly and reproducibly greatest in FVB/N males; lesser levels of these proteins were detected in B6 male (M) glomeruli but they were barely detectable or undetectable in female (F) glomeruli of either strain; glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as a housekeeping gene to assess loading. (b) Protein isolates from both undifferentiated (Undiff) and differentiated (Diff) podocytes contained CYP4A12A protein. Using immunohistochemistry, CYP4A12A was immunodetected in the glomeruli of tissue sections obtained from FVB/N male kidneys; some of the signal was found in podocytes as evidenced by double labeling with podoplanin (c–f, arrows). HSDS3B2 was also detected in the podocytes of the glomeruli (g–j, arrows). ACY3 was predominately immunolocalized to parietal glomerular epithelia, with a faint signal observed in glomerular tufts (k–n). (c–n) Bars=20 μm.