Figure 6.

Administration of testosterone to female B6 mice. Sustanon-250 injection for 4 weeks in 5-week-old B6 mice (n=6 in each group) led to a significant increase in (a) serum testosterone, (b) kidney/body weight, (c) albumin excretion rate, and (d) albumin/creatinine ratio. Despite these changes, blinded analysis of glomerular histology showed that there was no difference between B6 female mice injected with either (e) phosphate-buffered saline (PBS) or (f) Sustanon (SUS); (g) quantification is shown. (h) Stimulation of mouse podocytes in vitro (n=3 in each group) with Sustanon-250 led to a significant increase in Cyp4a12a mRNA levels; hypoxanthine-guanine phosphoribosyltransferase (hprt) was used as a housekeeping gene. (i) Acy3 mRNA tended to be elevated following Sustanon-250 exposure but this was not significant *P<0.05, **P<0.01; ***P<0.001 between groups. (e, f) Bars=30 μm. All data are presented as means and s.e.m. except c and d that were log transformed before analysis and are presented as geometric means and confidence interval.