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. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: J Mol Endocrinol. 2012 Oct 30;49(3):249–266. doi: 10.1530/JME-12-0097

Fig. 4.

Fig. 4

The assessment of the ligand-mediated mobility of GFP-ERβ (A) and GFP-ERβEBD (B) by FRAP. Transient transfection and processing of HeLa cells for FRAP were carried out as described in legend of Fig. 2. The normalized mean fluorescence recovery of GFP-ERβ species without (NL) or with a ligand from three independent experiments with a minimum of five individual cells per experiment was graphed without SEM, which was less than 15% of the mean.