Fig. 7.

The nuclear mobility of the chimeric ERα_Nβ_C in HeLa cells. Transiently transfected cells for 24h were incubated in the absence (NL) or presence of 10⁻⁹ M E2, 10⁻⁷ M 4HT or ICI for 1h. Cells were subjected to FRAP analysis as described in legend of Fig. 2. Graph represents the normalized mean fluorescence recovery of GFP- ERα_Nβ_C from three independent experiments with a minimum of five individual cells per experiment. SEM, which was less than 15% of the mean, is not shown for simplicity.