Table 1. Breakpoints of mtDNA deletions.
Break point sequences of Δ-mtDNA extracted from long range PCR on single respiratory deficient choroid plexus epithelium cells. Break points of Δ-mtDNA in MS were often flanked by repeat sequences (sequence in brackets, imperfect bases underlined), as reported previously in neurodegenerative diseases and ageing. Base pairs are shown pre- (5’) and post-breakpoint (3’).
| Break points | Repeat sequence | 5’ | 3’ |
|---|---|---|---|
| 6426-15,537 | No repeat sequence | AAAACCCCCTG | CCCCTCCCCACA |
| 6703-15,662 | No repeat sequence | AAAAAAAGAACC | ATCCTCCATATA |
| 7227-15,815 | perfect repeat | GACGTTACTCGG | (ACTA)TACTTCAC |
| 6420-16,033 | no repeat sequence | TC AATATAAAAC | GGGGAAGCAGAT |
| 6459-15,592 | imperfect repeat | CCCTCTTCGTCT | (GATCCGTCCCTAA) |
| 6474-15,733 | imperfect repeat | CCGTCCTAATCA | (CAGCAGTCCT)CA |
| 6455-14,865 | imperfect repeat | ACGCCCCTCTTC | (GTCTGATCC)TCC |
| 6826-13,071 | no repeat sequence | TTTCACCTCCGC | CCTACTCCACTC |
| 6646-15,911 | imperfect repeat | TATTCTTATCCT | (ACCAGGCTT)GTA |