Fig. 4.

GFP-NaPiIIa and mRFP-Shank2E expression characteristics in OK cells. A: Western blotting shows recombinant GFP-NaPiIIa and mRFP-Shank2E (closed arrowheads) are expressed at modestly higher levels than native NaPiIIa and Shank2 (open arrowheads). Actin served as a loading control (XF, transfected). B: confocal imaging in the X-Z (bottom rectangle) and Y-Z planes (right rectangle) shows that native NaPiIIa (green) and Shank2 (red) are concentrated at the apical domain. F-actin (blue) delineates the cell periphery. X-Y scans (top left box) are at the level of the subapical domain. C: confocal imaging shows that recombinant GFP-NaPiIIa (green) and mRFP-Shank2E (red) are similarly concentrated at the apical domain. Bar = 5 μm.