Table 1.

Migration coefficients for FCS, FCCS, and ccRICS studies

Proteins		Low Phosphate			High Phosphate
Protein1	Protein2	Dprotein1, μm2/s	Dprotein2, μm2/s	DFCCS, μm2/s	Dprotein1, μm2/s	Dprotein2, μm2/s	DFCCS, μm2/s	DccRICS, μm2/s
GFP	mCherry	9.5 ± 1.0 (n = 5)	34.5 ± 6.0 (n = 5)		28.9 ± 3.1 (n = 5)	99.3 ± 5.5 (n = 5)
NaPiIIa*	—	—	—		3.5 ± 0.6 (n = 5)	—
—	Shank2†	—	—		—	3.1 ± 0.5 (n = 5)
NaPiIIa*	Shank2†	—	—	—	3.9 ± 0.3 (n = 10)	3.8 ± 0.3 (n = 10)	3.7 ± 0.3 (n = 10)	2.5 ± 0.7 (n = 4)
Rab11*	NaPiIIa‡	5.8 ± 1.2 (n = 5)	—	—	7.6 ± 0.6 (n = 5)	3.3 ± 0.3 (n = 5)	—
Rab11*	Shank2†	6.0 ± 0.6 (n = 5)	—	—	6.8 ± 0.2 (n = 5)	3.0 ± 0.5 (n = 5)	—

Values are means ± SE. NaPiIIa, sodium-phosphate cotransporter IIa; D, migration coefficient; FCS, fluorescence correlation spectroscopy; FCCS, fluorescence cross-correlation spectroscopy ccRICS, raster image cross-correlation spectroscopy.

^*Proteins were GFP tagged;

^†proteins were mRFP-tagged;

^‡proteins were mCherry-tagged.