Figure 2.

Inverse correlation of RNA-257₁ and 1183 mRNA levels in the presence and absence of phosphate. (A) Northern blot analysis of RNA-257₁ levels with total RNA from Sso cells grown in either full medium (+P; 280 mg/l KH₂PO₄) or under phosphate-limiting conditions (−P). 5S rRNA was used as loading control. (B) qPCR analysis of RNA-257₁ and 1183 mRNA levels in the presence of phosphate (+P) and under phosphate-limiting conditions (−P). The Sso 0412 mRNA, encoding aIF2-γ, was used as a house-keeping endogenous control. The error bars represent s.d. from triplicate experiments. The numbers represent copies per 10 ng cDNA.