Figure 4.

Duplex formation between RNA-257₁ and 1183 mRNA augments degradation of 1183 mRNA in vitro. (A) Heat-inactivated Sso extracts and (B) ‘active’ S30 extracts were added to 1183 mRNA (lanes 1 and 3) or to the 1183 mRNA-RNA257₁ duplex (lane 2 and 4). After the indicated times in minutes, samples were withdrawn and the 1183 mRNA levels were determined with a probe specific for the 3′ end of 1183 mRNA (lane 1 and 2) and with a probe specific for the central part of 1183 mRNA (lane 3 and 4). The experiment was performed in triplicate. One representative experiment is shown. (C) Quantification of the results. The graph shows the relative amounts of the 3′ part of 1183 mRNA remaining in the absence of RNA-257₁ (blue) and when in duplex with RNA-257₁ (red) plotted as a function of time. The error bars represent s.d. from triplicate experiments.