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. 2013 Apr 26;14(6):568–576. doi: 10.1038/embor.2013.53

Figure 2.

Figure 2

Autophagy regulates developmental processes specified by the let-7 miRNA family and lsy-6 miRNA. (A–C) Wild-type and atg-2 hermaphrodites have a single vulva (arrow). (B) let-60(n1046gf) mutants show a Muv phenotype. Black arrows indicate pseudovulvae. (D) Loss of autophagy activity suppresses the Muv phenotype in let-60(n1046) mutants. The number of adult animals examined was: wild type (n=450), let-60 (n=406), epg-6; let-60 (n=444), let-60; atg-2 (n=803), epg-5; let-60 (n=672), epg-5; let-60; ain-1(ku322) (n=635). P values are determined by a two-tailed χ2 test. (E,F) lsy-6 mutants show defects in ASEL fate specification and do not express the ASEL-specific reporter lim-6pro::GFP. (G) Expression of lim-6pro::GFP in various strains. aCompared with lsy-6(ot150).bCompared with lsy-6(ot150); ain-1(ku322). P values are determined by a two-tailed χ2 test. (A–C) Scale bar, 20 μm; (E,F) scale bar, 10 μm. ASEL, ASE left; ASER, ASE right; GFP, green fluorescent protein; miRNA, microRNA; Muv, multivulva.