Table 1.
Sequences of primers used in this study.
| Gene | Primera | Product size |
|---|---|---|
| (bp) expected | ||
| stx1 | fp: 5′-ACGTTACAGCGTGTTGCRGGGATC-3′ | 121 |
| bp: 5′-TTGCCACAGACTGCGTCAGTRAGG-3′ | ||
| stx2 | fp: 5′-TGTGGCTGGGTTCGTTTATACGGC-3′ | 102 |
| bp: 5′-TCCGTTGTCATGGAAACCGTTGTC-3′ | ||
| eaeA | fp: 5′-TGAGCGGCTGGCATGATGCATAC-3′ | 241 |
| bp: 5′-TCGATCCCCATCGTCACCAGAGG-3′ | ||
| LT1 | fp: 5′-TGGATTCATCATGCACCACAAGG-3′ | 360 |
| bp: 5′-CCATTTCTCTTTTGCCTGCCATC-3′ | ||
| ST1 | fp: 5′-TTTCCCCTCTTTTAGTCAGTCAACTG-3′ | 160 |
| bp: 5′-GGCAGGACTACAACAAAGTTCACAG-3′ |
a
fp, forward primer; bp, backward primer. stxl and stx2: Shiga toxin1 and 2 encoding genes; eaeA: intimin encoding gene; LTl and STl: thermolabile and thermostable toxins encoding genes.