Table 3.
Phage stock/sensitive strain | Assay conditions | Log reduction in E. coli viable cellsa after the incubation time (h)b | |||
---|---|---|---|---|---|
T (°C) | MOI | 3 | 6 | 24 | |
DT1/EPEC (920) | 5 | 4.4 × 102 | NS | **0.80 ± 0.14 | NS |
24 | 4.8 × 102 | **0.30 ± 0.05 | NS | NS | |
5 | 4.4 × 101 | NS | **0.49 ± 0.09 | NS | |
24 | 4.8 × 101 | NS | NS | **0.46 ± 0.08 | |
DT6/EPEC (920) | 5 | 5.2 × 102 | **0.67 ± 0.12 | **0.59 ± 0.11 | *0.46 ± 0.15 |
24 | 6.5 × 103 | *0.32 ± 0.09 | NS | NS | |
5 | 5.2 × 101 | NS | *0.30 ± 0.08 | NS | |
24 | 6.5 × 102 | NS | NS | NS | |
DT6/non-O157 STEC (ARG4827) | 5 | 2.4 × 104 | *0.33 ± 0.09 | *0.47 ± 0.12 | *0.56 ± 0.17 |
24 | 4.0 × 102 | *0.43 ± 0.13 | NS | NS | |
5 | 2.4 × 103 | NS | *0.37 ± 0.09 | *0.50 ± 0.16 | |
24 | 4.0 × 101 | *0.35 ± 0.11 | NS | NS | |
DT6/O157:H7 STEC (464) | 5 | 2.3 × 103 | *0.59 ± 0.16 | **0.86 ± 0.15 | *0.38 ± 0.10 |
24 | 5.8 × 103 | **0.77 ± 0.14 | ***1.15 ± 0.12 | NS | |
5 | 2.3 × 102 | *0.38 ± 0.09 | *0.62 ± 0.18 | NS | |
24 | 5.8 × 102 | NS | **0.74 ± 0.13 | NS | |
Cocktail/DH5α | 5 | 2.25 × 104 | *0.91 ± 0.19 | **2.16 ± 0.20 | **2.23 ± 0.21 |
24 | 1.75 × 104 | *0.66 ± 0.15 | NS | NS | |
Cocktail/O157:H7 STEC (464) | 5 | 1.56 × 105 | NS | NS | NS |
24 | 3.33 × 105 | **1.43 ± 0.24 | **2.58 ± 0.21 | **2.20 ± 0.22 |
MOI, multiplicity of infection (PFU/CFU); NS, not significant. Mean values of treated and control samples not significantly different using the scheffé method (
significant at p = 0.05;
significant at p = 0.01;
significant at p = 0.001).
Log reduction in E. coli viable cells with respect to phage-free control.
Mean of three data points ± standard deviations.