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. 2013 Jun 6;3:20. doi: 10.3389/fcimb.2013.00020

Table 3.

E. coli viable cell logarithmic reductions after phage treatment of contaminated meat products.

Phage stock/sensitive strain Assay conditions Log reduction in E. coli viable cellsa after the incubation time (h)b
T (°C) MOI 3 6 24
DT1/EPEC (920) 5 4.4 × 102 NS **0.80 ± 0.14 NS
24 4.8 × 102 **0.30 ± 0.05 NS NS
5 4.4 × 101 NS **0.49 ± 0.09 NS
24 4.8 × 101 NS NS **0.46 ± 0.08
DT6/EPEC (920) 5 5.2 × 102 **0.67 ± 0.12 **0.59 ± 0.11 *0.46 ± 0.15
24 6.5 × 103 *0.32 ± 0.09 NS NS
5 5.2 × 101 NS *0.30 ± 0.08 NS
24 6.5 × 102 NS NS NS
DT6/non-O157 STEC (ARG4827) 5 2.4 × 104 *0.33 ± 0.09 *0.47 ± 0.12 *0.56 ± 0.17
24 4.0 × 102 *0.43 ± 0.13 NS NS
5 2.4 × 103 NS *0.37 ± 0.09 *0.50 ± 0.16
24 4.0 × 101 *0.35 ± 0.11 NS NS
DT6/O157:H7 STEC (464) 5 2.3 × 103 *0.59 ± 0.16 **0.86 ± 0.15 *0.38 ± 0.10
24 5.8 × 103 **0.77 ± 0.14 ***1.15 ± 0.12 NS
5 2.3 × 102 *0.38 ± 0.09 *0.62 ± 0.18 NS
24 5.8 × 102 NS **0.74 ± 0.13 NS
Cocktail/DH5α 5 2.25 × 104 *0.91 ± 0.19 **2.16 ± 0.20 **2.23 ± 0.21
24 1.75 × 104 *0.66 ± 0.15 NS NS
Cocktail/O157:H7 STEC (464) 5 1.56 × 105 NS NS NS
24 3.33 × 105 **1.43 ± 0.24 **2.58 ± 0.21 **2.20 ± 0.22

MOI, multiplicity of infection (PFU/CFU); NS, not significant. Mean values of treated and control samples not significantly different using the scheffé method (

*

significant at p = 0.05;

**

significant at p = 0.01;

***

significant at p = 0.001).

a

Log reduction in E. coli viable cells with respect to phage-free control.

b

Mean of three data points ± standard deviations.