Table 1.
List of primary and secondary antibodies used for immunostaining
| Primary antibody overnight incubations at room temperature |
Secondary antibody (2 h incubation) | Dilution | ||||||
|---|---|---|---|---|---|---|---|---|
| Antigen | Host | Dilution | Source | Catalog number | Immunogen | Specificity | ||
| GFP | Mouse | 1:500 | Invitrogen | A11120 | GFP isolated from Aequorea victoria | No staining in uninjected animals | Donkey anti-mouse alexa fluor 488 (Invitrogen) | 1:100 |
| GFP | Rabbit | 1:5000 | Invitrogen | A11122 | GFP isolated from Aequorea victoria | No staining in uninjected animals | Donkey anti-rabbit biotinylated | 1:500 |
| Cre | Rabbit | 1:10,000 | Novagen | 69050 | Bacteriophage P1qa Cre-recombinase, 35 kDa protein, native and denatured | Western blot showed binding to purified Cre recombinase; none detected in uninjected mouse brain | Donkey anti-rabbit biotinylated | 1:500 |
| Vglut2 | Rabbit | 1:500 | Synaptic Systems | 135403 | Strep-Tag fusion protein of rat Vglut2 (amino acids 510–582) | Preincubation of the Vglut2 antibody with immunogen peptide blocks labeling (Graziano et al., 2008). | Donkey anti-rabbit alexa fluor 546 (Invitrogen) | 1:100 |
| Neu-N | Mouse | 1:1000 | Millipore | MAB377 | Purified cell nuclei from mouse brain | Staining only of neuronal cell groups in brain, not blood vessels or meninges or in white matter | Donkey anti-rabbit biotinylated | 1:500 |