GFP |
Mouse |
1:500 |
Invitrogen |
A11120 |
GFP isolated from Aequorea victoria
|
No staining in uninjected animals |
Donkey anti-mouse alexa fluor 488 (Invitrogen) |
1:100 |
GFP |
Rabbit |
1:5000 |
Invitrogen |
A11122 |
GFP isolated from Aequorea victoria
|
No staining in uninjected animals |
Donkey anti-rabbit biotinylated |
1:500 |
Cre |
Rabbit |
1:10,000 |
Novagen |
69050 |
Bacteriophage P1qa Cre-recombinase, 35 kDa protein, native and denatured |
Western blot showed binding to purified Cre recombinase; none detected in uninjected mouse brain |
Donkey anti-rabbit biotinylated |
1:500 |
Vglut2 |
Rabbit |
1:500 |
Synaptic Systems |
135403 |
Strep-Tag fusion protein of rat Vglut2 (amino acids 510–582) |
Preincubation of the Vglut2 antibody with immunogen peptide blocks labeling (Graziano et al., 2008). |
Donkey anti-rabbit alexa fluor 546 (Invitrogen) |
1:100 |
Neu-N |
Mouse |
1:1000 |
Millipore |
MAB377 |
Purified cell nuclei from mouse brain |
Staining only of neuronal cell groups in brain, not blood vessels or meninges or in white matter |
Donkey anti-rabbit biotinylated |
1:500 |