Table 1.
Analysis of surface phenotype of splenocytes
| Cell Number/Spleen | |||||
|---|---|---|---|---|---|
| B cells |
Average Vehicle |
SEM Vehicle | Average PCI-32765 | SEM PCI-32765 | P-value |
| B220+ | 3.97E+07 | 1.13E+07 | 9.06E+06 | 2.76E+06 | * |
| Activated, B220+CD69+ | 3.71E+06 | 9.33E+05 | 5.29E+05 | 9.19E+04 | ** |
| T1, B220+AA4.1+CD23- | 1.64E+07 | 5.90E+06 | 2.95E+06 | 8.72E+05 | * |
| T2, B220+AA4.1+CD23+ | 1.60E+07 | 3.42E+06 | 4.02E+06 | 1.79E+06 | ** |
| Marginal zone, B220+AA4.1-CD23- | 1.99E+06 | 5.05E+05 | 5.25E+05 | 1.92E+05 | * |
| Follicular, B220+AA4.1-CD23+ | 5.13E+06 | 1.89E+06 | 8.82E+05 | 2.18E+05 | * |
| Plasmablast, B220+AA4.1-CD138+ | 3.62E+06 | 9.24E+05 | 4.36E+05 | 7.13E+04 | ** |
| Germinal center, B220+GL7+ | 8.61E+06 | 4.52E+06 | 8.72E+05 | 3.38E+05 | NS |
| B1-like, B220+CD5+ | 1.01E+06 | 2.62E+05 | 2.60E+05 | 3.93E+04 | * |
| Myeloid | |||||
| Myeloid DC, CD11b+CD11c+ | 1.33E+07 | 2.76E+06 | 2.19E+06 | 4.28E+05 | *** |
| Macrophage, CD11b+F4/80+ | 1.32E+07 | 3.11E+06 | 2.15E+06 | 3.88E+05 | ** |
| CD11b+Ly6C+ | 1.74E+06 | 5.54E+05 | 3.40E+05 | 6.72E+04 | * |
| Neutrophils, CD11b+Ly6G+ | 1.50E+07 | 3.51E+06 | 2.41E+06 | 4.16E+05 | ** |
| Mast cell, CD34+c-Kit+ | 3.74E+06 | 9.87E+05 | 7.36E+05 | 2.54E+05 | * |
| T cells | |||||
| CD4+ | 2.31E+07 | 4.00E+06 | 9.83E+06 | 9.76E+05 | ** |
| Activated, CD4+CD69+ | 8.29E+06 | 1.36E+06 | 2.64E+06 | 1.01E+06 | *** |
| Naïve, CD4+CD44-CD62L+ | 9.73E+05 | 2.34E+05 | 5.68E+05 | 9.45E+04 | NS |
| Central memory, CD4+CD44+CD62L+ | 5.11E+06 | 7.89E+05 | 2.84E+06 | 3.49E+05 | * |
| Effector memory, CD4+CD44+CD62L- | 1.56E+07 | 3.04E+06 | 6.00E+06 | 7.58E+05 | ** |
| CD8+ | 2.01E+07 | 3.86E+06 | 5.29E+06 | 8.23E+05 | ** |
| Activated, CD8+CD69+ | 8.57E+06 | 1.60E+06 | 2.54E+06 | 1.17E+06 | ** |
| Naïve, CD8+CD44-CD62L+ | 6.18E+03 | 3.33E+03 | 3.34E+03 | 2.65E+03 | NS |
| Central memory, CD8+CD44+CD62L+ | 5.64E+06 | 1.38E+06 | 9.08E+05 | 1.65E+05 | ** |
| Effector memory, CD8+CD44+CD62L- | 1.45E+07 | 2.87E+06 | 4.38E+06 | 8.23E+05 | ** |
Quantitative analysis of B6.Sle1.Sle3 splenic cell populations as determined by flow cytometry. All data are expressed as the mean (Average) and standard error of the mean (SEM) of the data for the PCI-32765-treated (n = 9) or vehicle-treated (n = 8) splenocytes, which were compared by two-tailed Student's t-test. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.005, compared to vehicle; NS, not significant.