Table 3.
Comparison of visual and automated interpretation of IIF ANCA patterns of the 'test set' patients (n = 342) on ethN and formN.
| ethN | Automated interpretation by AKLIDES™ | ||||
| n (%) | cytoplasmic | nuclear | atypical | negative | |
| cytoplasmic | 59 (17.2) | 0 (0) | 7 (2.0) | 22 (6.4) | |
| nuclear | 0 (0) | 61 (17.8) | 4 (1.2) | 0 (0) | |
| atypical | 3 (0.9) | 10 (2.9) | 40 (11.7) | 2 (0.6) | |
| negative | 0 (0) | 0 (0) | 1 (0.3) | 133 (38.9) | |
| Visual interpretation | formN | ||||
| n (%) | cytoplasmic | atypical | negative | ||
| cytoplasmic | 91 (26.6) | 0 (0) | 9 (2.6) | ||
| atypical | 8 (2.3) | 0 (0) | 0 (0) | ||
| negative | 9 (2.6) | 0 (0) | 225 (65.8) | ||
ANCA IIF patterns were first read automatically with AKLIDES™ followed by manual interpretation with a routine fluorescence microscope. Both interpretation methods demonstrated good agreement on ethN and formN (κ = 0.746, 95% CI: 0.667 to 0.825; κ = 0.847, 95% CI: 0.79 to 0,904; respectively). ANCA, antineutrophil cytoplasmic antibody; ethN, ethanol-fixed neutrophils; formN, formalin-fixed neutrophils; IIF, indirect immunofluorescence.