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. Author manuscript; available in PMC: 2013 Jun 6.
Published in final edited form as: Biochem J. 2011 Oct 1;439(1):45–55. doi: 10.1042/BJ20110274

Figure 3. ATX-V5 increases LPA levels and cell migration.

Figure 3

A) A549 cells were incubated with serum free medium from empty virus infected A549 cells (Cont Med) or from adenovirus encoding ATX-V5 (1-10 MOI, 24 h) infected A549 cells (ATX-V5 Med) and cell migration was measured at 18 h by scratch and transwell migration assay. The data represents mean ± S.D. from three independent experiments. *p<0.05, compared to vector control medium in scratch assay; *p<0.01, compared to vector control medium in transwell assay B) HSAEpCs were incubated with serum free Cont Med or ATX-V5 Med for 18 h, and cell migration was measured by scratch assay at 18 h. The data represents mean ± S.D. from three independent experiments. *p<0.01, compared to vector control medium. C) A549 cells were incubated with serum free Cont Med or ATX-V5 Med with or without egg LPC (0 – 200 μM), and cell migration was measured at 18 h by scratch assay and transwell migration assay (D). The data represents mean ± S.D. from three independent experiments. *p<0.05, compared to control medium in scratch assay; *p<0.05, compared to control medium in transwell assay. E) ATX-V5 Med was incubated with an antibody to V5 tag or IgG control (10 μg/ml) overnight, and then was added to scratched cells. After 18 h, migration was measured. The data represents mean ± S.D. from three independent experiments. *p<0.05, compared to Cont Med-treated cells; **p<0.05, compared to ATX-V5 Med alone or plus control IgG-treated cells. F) A549 cells were treated with Cont Med or ATX-V5 Med for 18 h and actin microfilaments were immunostained by Texas Red-labeled phalloidin for 1 h. Shown are representative images from three independent experiments.