Skip to main content
. Author manuscript; available in PMC: 2013 Jun 6.
Published in final edited form as: Biochem J. 2011 Oct 1;439(1):45–55. doi: 10.1042/BJ20110274

Figure 8. LPS increases ATX levels in lung fluid.

Figure 8

A). Sv/129 mice were intratracheally challenged with PBS (n=3) or LPS (5 mg/kg body weight, n=4) for 24 h, and lung tissues were stained with H&E. Shown are representative images from 3-4 mouse tissues. B) Total RNA was extracted from lung tissue and ATX mRNA levels were measured by Real-time PCR. The data represents mean ± S.D. from 3-4 lung samples. C) BAL fluid from control and LPS challenged mice were analyzed by Western blotting with an antibody to ATX. Numbers indicate the number of mice used in each group. D) LPA levels in BAL fluids were measured by LC/MS/MS. The data represents mean ± S.D. from 3-4 samples. *<0.01, compared to PBS.