Figure 6.
H3K79 and H3K4 are hypermethylated on the Hoxa locus in CALM-AF10 and NESCALM-AF10 leukemias. ChIP analysis of di-me H3K79 (A) and trimethylated H3K4 (B) in the promoter regions of the Hoxa cluster genes. Hoxa amplification was measured by real-time PCR as a percent of input then normalized to Hoxa9/Meis1 control leukemic cells. Black bars represent CALM-AF10, checkered bars are NESCALM-AF10, and white bars are Hoxa9/Meis1 leukemias. Results are shown as mean ± SEM compiled from 3 separate leukemias.