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Hydrolysis of LN-derived phospholipids by recombinant PLA2G2D. (A–C) ESI-MS of phospholipids (A), lysophospholipids (B), and fatty acids (C) in total lipids extracted from the LNs and incubated with (+) or without (−) 200 nM recombinant mouse PLA2G2D (mean ± SEM, n = 3 per group). (D) ESI-MS of LA, AA, and DHA in the LNs of WT mice (mean ± SEM from five mice). Results in A–D are representative of three independent experiments. *, P < 0.05; **, P < 0.01.
