Table 1.
Callus induction and rhizogenesis from root explants of Dianthus caryophyllus cultured on an MS medium supplemented with various hormones after 6 months of culture.
MS media + hormone (mg L−1) | Rhizogenesis | Callus formation (%) | Colour of callus | Observations | ||
---|---|---|---|---|---|---|
NAA | BAP | % | Mode | |||
0.0 | 0.0 | NR | NR | NR | N/A | Necrotic |
| ||||||
0.5 | 0.0 | 57.50f | Indirect | 20.00a | White | Friable callus Roots formed after 8 days |
| ||||||
1.0 | 0.0 | 100.00g | Indirect | 100.00m | White | Friable callus Roots formed after 7 days |
| ||||||
1.5 | 0.0 | 100.00g | Indirect | 90.00l | White | Friable callus Roots formed after 7 days |
| ||||||
2.0 | 0.0 | 100.00g | Indirect | 100.00m | White | Friable callus Roots formed after 7 days |
| ||||||
0.0 | 0.5 | NR | N/A | NR | N/A | Necrotic after 6 weeks |
1.0 | NR | N/A | NR | N/A | Necrotic after 6 weeks | |
1.5 | NR | N/A | NR | N/A | Necrotic after 6 weeks | |
2.0 | NR | N/A | NR | N/A | Necrotic after 6 weeks | |
| ||||||
0.5 | 0.5 | 5.00a | Indirect | 35.00d | White | Friable callus with roots |
1.0 | NR | N/A | 43.00e | White | Friable callus with roots | |
1.5 | NR | N/A | NR | N/A | Necrotic after 7 weeks | |
2.0 | NR | N/A | 25.00b | White | Friable callus with roots | |
| ||||||
1.0 | 0.5 | 10.00b | Indirect | 55.00g | White | Friable callus with roots |
1.0 | NR | N/A | 82.00j | White | Friable callus with roots | |
1.5 | NR | N/A | 20.00a | White | Friable callus with roots | |
2.0 | NR | N/A | NR | N/A | Necrotic after 5 weeks | |
| ||||||
1.5 | 0.5 | NR | N/A | 100.00m | White and green | Friable callus |
1.0 | NR | N/A | 75.00h | White and green | Friable callus | |
1.5 | NR | N/A | 80.00i | White and green | Friable callus | |
2.0 | 45.00e | Indirect | 30.00c | White | Friable callus with roots | |
| ||||||
2.0 | 0.5 | NR | N/A | 75.00h | White and green | Friable callus |
1.0 | 30.00d | Indirect | 85.00k | White | Friable callus with roots | |
1.5 | NR | N/A | 50.00f | Green | Compact callus | |
2.0 | NR | N/A | 30.00c | White and green | Friable callus | |
| ||||||
3.0 | 1.0 | 10.00b | Direct | NR | N/A | Necrotic after 6 weeks |
3.0 | 25.00c | Direct | 30.00c | N/A | Friable callus with roots |
*Means with different letters in the same column differ significantly at P < 0.05 by one-way ANOVA and Duncan's multiple range test.
(NR: no response, N/A: not available).