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. Author manuscript; available in PMC: 2013 Jun 6.
Published in final edited form as: Methods Mol Biol. 2012;796:117–132. doi: 10.1007/978-1-61779-334-9_7

Fig. 2.

Fig. 2

Protein crosslinking. Protein side chains react with bifunctional reagents to form mono-derivatized and cross-linked forms of interacting proteins. Subclasses within each form simultaneously occur through different combinations of mono-derivatization and crosslinking. In the continuous process of crosslinking, conjugates of increasing size may also be formed, progressing from dimers, tetramers, etc. to large extensively crosslinked polymers.