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. Author manuscript; available in PMC: 2014 May 1.
Published in final edited form as: Mol Microbiol. 2013 Apr 14;88(4):740–753. doi: 10.1111/mmi.12219

Figure 5.

Figure 5

A. M. xanthus strains in which FrzZ was replaced by fluorescently tagged FrzZ-GFP show phenotypes similar to strains expressing untagged FrzZ. Images show motility behavior on soft agar surface after 48 h incubation (top), as well as fruiting body formation under starvation conditions after 96 h (bottom). Shown is the colony edge of wild type and selected hypo-reversing (ΔfrzE), hyper-reversing (frzCDc) and non-motile (ΔmglA) strains that express FrzZ-GFP. Scale bars represent 2 mm. See supplementary Fig. S1 for phenotypic analysis of all fluorescent strains used in this study. B. Phosphorylation analysis of selected M. xanthus strains expressing FrzZ-GFP (57.3 kD). Cell extracts were analyzed by Phos-tag (50 µM) SDS-PAGE and Western Blot using anti-FrzZ antibody.