. Author manuscript; available in PMC: 2014 May 1.

Published in final edited form as: Mol Microbiol. 2013 Apr 14;88(4):740–753. doi: 10.1111/mmi.12219

Table 1.

plasmids	relevant characteristics	Reference
pBJ114	gal^S kan^R, for the construction of insertion and deletion mutations in M. xanthus	(Julien et al., 2000)
pSWU30	tet^R, integrates at Mx8 phage attachment site attB	(Wu & Kaiser, 1995)
pMR3562	tet^R, integrates at 1.38 kb site into the M. xanthus chromosome	(Iniesta et al., 2012)
pEGFP-C1	contains coding sequence for green fluorescent protein	Clontech
pSWU19mglBY	mglB-yfp; kan^R, integrates at Mx8 attachment site	(Zhang et al., 2010)
pEMC68	pBJ114 with frzZ bp 267-end to fused to GFP	this study
pCK121	pBJ114 with frzZ^{D52E D220E} bp 267- end fused to GFP	this study
pCK126	pSWU30 mglB-mCherry	this study
pCK133	pMR3562 mglA-mCherry	this study
oligonucleotides
EMC32	5’-ATGGTGAGCAAGGGCGAGGAG-3’
EMC35	5’-CCTCGCCCTTGCTCACCATCTCGTTACCGGTGGGCATC-3’
EMC36	5’-CGGGATCCCTACTTGTACAGCTCGTCCATG-3’
EMC37	5’-CGGAATTCCGGAAAGACGAAGGCGTCG-3’
C221	5’-GCAAGCTTTTACTTGTACAGCTCGTCCATG-3’
C243	5‘-GCCTCGAGCTCTGGCGCGCCGGGCATGGTGAGCAAGGGCGAG-3‘
C259	5’-GCGAATTCGTGGGAAGGGCTCTTTCAG-3’
C272	5’-GCCATATG TCCTTCATCAATTACTCATC-3’
C273	5’-GCGAATTCTCACCACCCTTCTTGAGCTCGG-3’
C269	GCGAATTCCTCTGGCGCGCCGGGCATGGTGAGCAAGGGCGAG-3’
C270	5’-GCGCTAGCTTACTTGTACAGCTCGTCCATG-3’
E. coli DH5α	host for plasmid construction	Invitrogen
M. xanthus strains
DZ2	wild type	(Campos et al., 1978)
DZ4484	ΔfrzZ	(Bustamante et al., 2004)
DZ4706	frzZ^{D52E D220E}	(Inclan et al., 2007)
DZ4704	frzZ^D52E	(Inclan et al., 2007)
DZ4705	frzZ^D220E	(Inclan et al., 2007)
DZ4481	ΔfrzE	(Bustamante et al., 2004)
DZ4480	ΔfrzCD	(Bustamante et al., 2004)
DZ4487	frzCD^c	(Bustamante et al., 2004)
DZ4547	frzE^ΔcheY	(Li et al., 2005)
DZ4629	frzE^H49A	(Inclan et al., 2008)
DZ4558	frzCD^c frzE^ΔcheY	laboratory collection
DZ4469	ΔpilA	(Vlamakis et al., 2004)
DZ4846	ΔagmU	Beiyan Nan
DZ4771	ΔpilAΔagmU	(Nan et al., 2010)
TM12	ΔmglA	(Mauriello et al., 2010)
DZ4832	frzCD^c frzE^ΔcheYΔfrzZ^{D52E D220E}	this study
DZ4833	frzZ-gfp kan^R	this study
DZ4834	frzCD^c frzZ-gfp kan^R	this study
DZ4835	ΔfrzE frzZ-gfp kan^R	this study
DZ4836	ΔfrzCD frzZ-gfp kan^R	this study
DZ4837	ΔmglA frzZ-gfp kan^R	this study
DZ4838	ΔmglB frzZ-gfp kan^R	this study
DZ4839	frzZ^D52E-gfp kan^R	this study
DZ4840	frzZ^D220E-gfp kan^R	this study
DZ4841	frzZ^{D52E D220E}-gfp kan^R	this study
DZ4842	ΔagmU frzZ-gfp kan^R	this study
DZ4843	ΔpilA frzZ-gfp kan^R	this study
DZ4844	ΔpilA ΔagmU frzZ-gfp kan^R	this study
DZ4845	frzZ-gfp attB::mglB-mCherry kan^R tet^R	this study
DZ4847	frzZ-gfp 1.38kB::mglA-mCherry kan^R tet^R	this study