Table3.

Inactivation kinetics of hPIEZO1 mutations

Mutation	Inactivation kinetics			Imaxat −80 mV
	n	Threshold (μm)	Inactivation time constant τ (ms)	n	nA
hPIEZO1	25	5.0 ± 0.3	8.6 ± 0.4	9	2.2 ± 0.4
hPIEZO1-R1358P	12	4.3 ± 0.5	13.8 ± 1.5***	12	1.4 ± 0.3
hPIEZO1-A2020T	19	4.7 ± 0.2	12.2 ± 0.5***	8	2 ± 0.4
hPIEZO1-T2127M	10	5.0 ± 0.5	11 ± 0.7*	10	2.2 ± 0.8
hPIEZO1-M2225R	16	4.4 ± 0.4	13.6 ± 1.1***	9	7.7 ± 3.7
hPIEZO1-R2456H	14	4.7 ± 0.4	22.2 ± 2.1***	7	1.6 ± 0.5
hPIEZO1-E2496ELE	16	4.2 ± 0.3	15 ± 1.1***	11	1.5 ± 0.2

All values measured from cells held at −80 mV membrane potential.

Average values expressed as mean ± s.e.m.

^*p< 0.05;

^***p < 0.001 relative to WT hPIEZO1 (Student’s t-test).