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. 2013 Mar 3;22(13):2553–2565. doi: 10.1093/hmg/ddt103

Figure 5.

Figure 5.

Middle ear histology in Tgif homozygous mice. Each group of panels consists of representative images (transverse sections) of the middle ears of homozygous (Tgif/Tgif) and wild-type (+/+) mice at different time points. (A and B) Five DAB (mutants analysed n = 8, controls analysed n = 5); (C and D) 14 DAB (mutants n = 8, controls n = 10); (E–H) 21 DAB (mutants n = 6, controls n = 16); (I–L) 1-month old (mutants n = 11, controls n = 7); (M–T) 2 months old (mutants n = 12, controls n = 7); (U–X) 4 months old (mutants n = 10, controls n = 10). (F and G) enlarged views of E; (J and K) enlarged views of I; (N and P) enlarged views of M; (R and S) enlarged views of Q; (V and W) enlarged views of U. (F) Inset viable and apoptotic PMNs. (O) Immunohistochemical staining of a 2-month-old homozygous mouse ear section using an F4/80 antibody. Macrophages are indicated with an arrow. Where indicated, the thickness of the epithelial lining is shown with a double arrow. The presence of cellular aggregates is indicated by an asterisk (P) and an inflamed polyp by an arrowhead (V). A–E, I, M, Q, U scale bar = 4 mm; F, J, N, O, R scale bar = 100 μm; G, H, K, L, P, S, T, V, W, X scale bar = 200 μm. Pie charts represent percentage of homozygote mice from different time points with no effusion and with effusion in one or in both ears.