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Reconstitution of nucleosomes. (A) Positive MALDI-TOF mass spectrum of histone octamers purified by FPLC. (B) Native 5% PAGE of nucleosome core particles (lane 1) and free 147 base pair-long 601 DNA containing the reporter sequence (lane 2) in 0.3×TBE buffer. (C) Histograms of hydroxyl radical footprints of nucleosomes and of free DNA.
