Figure 6.

Relative yields of oxidatively generated damage at the three different GGG-sites initiated by hole injection in nucleosomal and in free 5′-³²P-end-labeled 2AP-modified DNA strands. The oxidatively modified guanine bases were transformed to DNA strand breaks by either (i) standard hot piperidine treatment, or (ii) by incubation with Fpg-protein followed by digestion of histone proteins with Proteinase K, or (iii) by digestion of histone proteins with Proteinase K before incubation with Fpg-protein. The yields calculated from the integrated peak areas in the histograms represent the mean of four independent experiments.