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FCS binding curve of MET928 titrated against InlB-ATTO647N. The diffusion time τD of the complex is plotted against the receptor concentration. The binding curves are fitted by a Langmuir 1:1 binding model. The diffusion time of InlB-ATTO647N in the absence of MET is marked as green line. The inflection point represents the dissociation constant Kd = 5.0 ± 0.8 nM.
