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. 2013 May 30;13:268. doi: 10.1186/1471-2407-13-268

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Copyright © 2013 Blanchard et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Subcellular localization of claudin 1 protein in BT-20 cells. Subcellular fractions of control BT-20 cells were analyzed by Western blot using the claudin 1 antibody. A. Short exposure shows claudin 1 in the membrane fraction only, B. longer exposure reveals some protein in the cytoskeletal and to a lesser extent, the nuclear fraction. The arrow indicates the 21kD claudin 1 protein. C. Immunofluorescent staining with the claudin 1 antibody (left panels) shows positive fluorescence for claudin 1 in the cell membrane and the cytoplasm of a control clonal cell line and reduced fluorescence in the claudin 1 knockdown clone (clone 3).