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Knockdown of claudin 1 protein in stably transfected BT-20 HBC cells. Cells were transfected with a control sequence (C1, C2) and two different shRNA constructs targeting claudin 1 (3,4). The shRNA sequence 4 shows partial knockdown whereas sequence 3 shows >90% knockdown of the claudin 1 protein. (β-actin loading control; Western blot analysis; Cldn1 = claudin 1).
