Table 1. Significance analysis examining quadruplicate binding measurements of SIINFEKL fragments.
Peptide | p-value < | Relative binding mean (σ) |
EKL | 0.9 | - |
FEKL | 0.5 | - |
FEK | 1 | - |
NFEKL | 1 | - |
NFEK | 0.9 | - |
NFE | 0.9 | - |
INFEKL | 0.001 | 7% (2%) |
INFEK | 0.4 | - |
INFE | 1 | - |
INF | 0.7 | - |
IINFEKL | 0.001 | 22% (1%) |
IINFEK | 0.8 | - |
IINFE | 0.9 | - |
IINF | 0.9 | - |
IIN | 0.5 | - |
SIINFEK | 0.001 | 5% (1%) |
SIINFE | 0.001 | 4% (0%) |
SIINF | 0.001 | 8% (3%) |
SIIN | 0.9 | - |
SII | 0.9 | - |
SIINFEKL | 0.001 | 100% (1%) |
NoLigand | 0.5 | - |
The null hypothesis for the Welch-test [28] considers the distribution of respective peptide fragments measurements to not be greater than the distribution of NoLigand measurements.