Figure 7. Expression of both nascent and mature mRNA of target genes is reduced in Nxt1 mutant spermatocytes.

(A) Expression of nascent (unspliced) RNA was quantified using primer pairs a+c, b+c or b+d, while mRNA levels were determined with primer pairs a+sr or sf+d. (B) CG12699 and ocn control genes were expressed at broadly similar levels in WT and mutant testes, while expression of both nascent and mature RNA for the Nxt1-dependent test genes CG4907, CG10478, CG11249, CG14546, CG16736, CG17380, CG32487, CG33125 and pif2 were reduced. For each gene, the expression levels of nascent transcript (dark gray) and mature transcript (light gray) in Nxt1^z2-0488/Nxt1^DG05102 are presented as relative levels to those found in the wild-type (arbitrarily assigned as 1).