Table 2. QTLs identified for maximal ethanol accumulation capacity (pool 1, 22 segregants) by pooled-segregant whole-genome sequencing.

QTL	Chr.	Genomic position (bp)	Nr. SNPs with significant linkage	Average Probability of linkage	Association with parent	Presence in pool 2
2	I	168455–179051	30	0.996868	Seg5	No
3	V	69939–166080	348	0.999346	Seg5	No
4	V	178671–198538	84	0.999191	Seg5	No
5	V	230340–269314	187	0.997819	Seg5	No
7	X	136210–175751	148	−0.986817	BY	No
8	X	288210–321763	107	0.999024	Seg5	No
9	X	486491–594119	230	0.99672	Seg5	No
10	XII	1022570–1053429	94	−0.999094	BY	Weak
12.1	XIII	109860–137864	47	0.994056	Seg5	Yes
13	XIII	346583–352695	27	0.991967	Seg5	Weak
17.1	XV	372007–494421	247	−0.999883	BY	Yes

Eight QTLs were associated with the genome of the superior parent Seg5 and three QTLs linked to the genome of the inferior parent BY710. The chromosomal position of each QTL, the number of SNPs with significant linkage and the average Probability of linkage of all significant SNPs in the QTL are indicated. All QTLs indicated had a significant Probability of linkage >0.95 when linked to the Seg5 parent or <−0.95 when linked to the BY parent. QTLs 1, 6, 11, 14, 15 and 16 were found only in pool 2 (see Table 3) whereas QTLs 12 and 17 were common for both pools and designated 12.1 and 12.2 or 17.1 and 17.2 depending on the pool.