Table 3. QTLs identified for tolerance of cell proliferation to high ethanol (pool 2, 32 segregants) by pooled-segregant whole-genome sequencing.

QTL	Chr.	Genomic position (bp)	Nr. SNPs with significant linkage	Average Probability of linkage	Association with parent	Presence in pool 1
1	I	29970–55793	83	-0.998124	BY	Weak
6	VII	585062–600706	50	0.99851	Seg5	Weak
11	XIII	43152–51596	37	0.97562	Seg5	Weak
12.2	XIII	79761–173678	183	0.998144	Seg5	Yes
14	XIV	525370–549448	70	0.997764	Seg5	No
15	XV	161704–184072	59	0.997942	Seg5	Weak
16	XV	205844–210327	26	0.970977	Seg5	Weak
17.2	XV	356119–487809	285	-0.99949	BY	Yes

There are six QTLs linked to the genome of the superior parent Seg5 and two QTLs linked to the genome of the inferior parent BY710. The chromosomal position of each QTL, the number of SNPs with significant linkage and the average Probability of linkage of all significant SNPs in the QTL are indicated. All QTLs indicated had a significant Probability of linkage >0.95 when linked to the Seg5 parent or <−0.95 when linked to the BY parent. QTLs 2, 3, 4, 5, 7, 8, 9, 10 and 13 were found only in pool 1 (see Table 2) whereas QTLs 12 and 17 were common for both pools and designated 12.1 and 12.2 or 17.1 and 17.2 depending on the pool.