Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 Jun 6;9(6):e1003538. doi: 10.1371/journal.pgen.1003538

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2013 Carofiglio et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

(A–C) The number of RAD51 foci decreases from leptotene to zygotene in Spo11^+/+ and Spo11^+/YF spermatocytes, whereas a few foci are detected in Spo11^YF/YF spermatocytes and oocytes at both stages. (A–B) Double immunostaining with anti-SYCP3 (red), anti-RAD51 (green) of spermatocyte (A) and oocyte (B) nuclei from Spo11^+/+ (A–B, left panel) and Spo11^YF/YF (A–B, right panel) mice. Arrowheads point to RAD51 foci in Spo11^YF/YF spermatocytes and oocytes, both leptotene and zygotene. Extensive accumulation of RAD51 along axial elements of one or few chromosomes (arrows) can be observed in both Spo11^+/+ and Spo11^YF/YF oocyte nuclei (B, lower panel). Size bars represent 10 µm. (C) The number of RAD51 foci was counted in Spo11^+/+, Spo11^+/YF, and Spo11^YF/YF leptotene and zygotene spermatocytes and oocytes. Each dot represents the focus count of one nucleus. Black bars indicate mean number of foci. P values for the indicated comparisons (Mann-Whitney, two-tailed), and genotypes are indicated in the plot. (D) The number of MLH1 foci in pachytene spermatocyte nuclei was counted in Spo11^+/+and Spo11^+/YF mice. Black bars indicate the mean values. (E) Number of RAD51 foci at E17.5 in Spo11^+/+ and Spo11^YF/YF oocytes.