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. 2013 Jun 6;9(6):e1003535. doi: 10.1371/journal.pgen.1003535

Figure 2. JH and ILP2 regulate carbohydrate, protein, and lipid metabolism during starvation.

Figure 2

A. Total carbohydrate, protein, and lipid levels were determined by Anthrone reagent, Bradford, and vanillin reagent respectively in samples collected from day 3 to day 8 starved and fed male beetles. Three beetles were used for each time point and six biological replicate were used. The Means+S.D (n = 6) are shown. B. The nutrient levels including carbohydrate, protein, and lipid are regulated by JH and ILP2 during starvation. The male beetles injected with malE, ILP2, or JHAMT dsRNA were collected on day 8. The total carbohydrate, protein, and lipid were determined. Shown are the Means+S.D (n = 6). Asterisks show treatments that are significantly different (P<0.05) by one-way ANOVA.