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. 2013 Jun 6;9(6):e1003535. doi: 10.1371/journal.pgen.1003535

Table 1. Primers used for dsRNA and real time PCR.

Genes Primer sequence(5′-3′)
TRET-qRT-PCR F TAATCGATCGTTGTGGGAGACGCT
(G13653) R TAGGCTGCAGTATTTCGCCCAAGT
TRET-dsRNA* F GGAGCCGTCAACTTTGCATCAACA
(G13653) R TCTCCAACCACGTCCTTGAACAGT
trehalase-qRT-PCR F GCGCTCCAACTACAAAGCGTTCAA
(G04791) R TCCATGATGATCCCGTTCGTCCAA
trehalase-dsRNA* F ACCCGACTAATATTGCGCCACTGT
(G04791) R CGTGTTGTTCAGGCCCACAATCAT
TPS-qRT-PCR F GCAACTTTGACAATGTCGTCGCCT
(G07883) R ACCCACCCATAAGCCATTACCGTT
TPS-dsRNA* F AAGGCGTTGAAGTCTCTCCCGAAA
(G07883) R TTTACGGTCGACACGACATCCCAA
*

The T7 promoter sequence was added at the 5′ end of each dsRNA primer.