Table 1. Primers used for dsRNA and real time PCR.
Genes | Primer sequence(5′-3′) | |
TRET-qRT-PCR | F | TAATCGATCGTTGTGGGAGACGCT |
(G13653) | R | TAGGCTGCAGTATTTCGCCCAAGT |
TRET-dsRNA* | F | GGAGCCGTCAACTTTGCATCAACA |
(G13653) | R | TCTCCAACCACGTCCTTGAACAGT |
trehalase-qRT-PCR | F | GCGCTCCAACTACAAAGCGTTCAA |
(G04791) | R | TCCATGATGATCCCGTTCGTCCAA |
trehalase-dsRNA* | F | ACCCGACTAATATTGCGCCACTGT |
(G04791) | R | CGTGTTGTTCAGGCCCACAATCAT |
TPS-qRT-PCR | F | GCAACTTTGACAATGTCGTCGCCT |
(G07883) | R | ACCCACCCATAAGCCATTACCGTT |
TPS-dsRNA* | F | AAGGCGTTGAAGTCTCTCCCGAAA |
(G07883) | R | TTTACGGTCGACACGACATCCCAA |
The T7 promoter sequence was added at the 5′ end of each dsRNA primer.