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. 2013 Jun 6;8(6):e65519. doi: 10.1371/journal.pone.0065519

Figure 5. Establish non-toxic MTX and MMF dose regimens for in vivo selection.

Figure 5

6–8 week old NSG mice (n = 6) were (a), administered MTX by i.p. injection at 25 mg/kg/day twice a week the first week (days 1 and 4) and only once the second week (day 8), and/or (b), fed 0.563% MMF medicated feed for 2 weeks. Serum MTX and MPA levels in each case were analyzed by HPLC. Drug toxicity was then monitored by measuring (c), white blood cell counts, (d), body weight, (e), Hemoglobin, (f), ALT, and (g), creatinine levels. (c–g), Mean levels of each measurement (± S.D) after the last treatment (i.e., at day 14) are depicted. There was no significant difference between control and treatment mice (p≥0.05). Data were evaluated between control and treatment mice using an unpaired, two-tailed Student’s t - test.