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. 2013 Jun 6;8(6):e65716. doi: 10.1371/journal.pone.0065716

Figure 3. Recovery of non-O157 STEC strains from enrichment samples with different RT-PCR Ct values for stx.

Figure 3

The fraction of samples positive for isolation of at least one non-O157 STEC strain based on threshold PCR Ct value (M1) is compared to the fraction positive with both the PCR and IMS methods (M2). A Ct <27 was selected as the value required for routine plating of an enrichment broth on C-O157. However, all enrichment broths, regardless of Ct value for stx, were treated with O157-IMS and beads were plated on NT-RA(IMS method) for attempted recovery of non-O157 STEC strains (M2).