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. 2013 Jun 6;8(6):e65716. doi: 10.1371/journal.pone.0065716

Table 1. Primers and probes.

Name Sequencea Positionb
Stx1 forward CATCGCGAGTTGCCAGAAT 803
Stx1 reverse TCCCACGGACTCTTCCATCT 874
Stx1 probe Q670-ATCTGATGATTTCCTTCTATGTGTCCG-BHQ2 825
Stx2abc forward GGACCACATCGGTGTCTGTTATT 167
Stx2abc reverse CCCTCGTATATCCACAGCAAAAT 234
Stx2abc probe CFO560-CCACACCCCACCGGCAGT-BHQ1 192
Stx2ex forward GAAACTGCTCCTGTTTATACGATGAC 616
Stx2ex reverse CCGGAAGCACATTGCTGAT 697
Stx2ex probe FAM-CCCCCAGTTCAGAGTGAGGTCCACG-BHQ1 675
Stx2f forward CGCTGTCTGAGGCATCTCC 608
Stx2f reverse TCCTCTGTACTCTGGAAGAACATTAC 708
Stx2f probe CFR610-TTATACAATGACGGCTCAGGATGTTGACCTTACC-BHQ2 630
ompA forwardc CAGTGATGCGAATCTATGCCAAGATAACACCTGGTACACTGG 72
ompA reversec TGCCAATCGCATGCTTTGCCTATGTGTGCGTCACCGATGTTG 517
seq ompAf CAGTGATGCGAATCTATGCCA NA
seq ompAr TGCCAATCGCATGCTTTGCCTA NA
a

Fluorescent probe labels are Q670, Quasar 670; CFO560, CAL Fluor Orange 560; FAM, Carboxyfluorescein and CFR610, CAL Fluor Red 610. Quenchers are BHQ, Black Hole Quencher 1 and 2 (Biosearch, Novato, CA).

b

Position is relative to the coding region of stx1, stx2A or ompA from EDL933.

c

ompA forward and reverse sequences in BOLD are homologous to ompA.