Table 1. Primers and probes.
Name | Sequencea | Positionb |
Stx1 forward | CATCGCGAGTTGCCAGAAT | 803 |
Stx1 reverse | TCCCACGGACTCTTCCATCT | 874 |
Stx1 probe | Q670-ATCTGATGATTTCCTTCTATGTGTCCG-BHQ2 | 825 |
Stx2abc forward | GGACCACATCGGTGTCTGTTATT | 167 |
Stx2abc reverse | CCCTCGTATATCCACAGCAAAAT | 234 |
Stx2abc probe | CFO560-CCACACCCCACCGGCAGT-BHQ1 | 192 |
Stx2ex forward | GAAACTGCTCCTGTTTATACGATGAC | 616 |
Stx2ex reverse | CCGGAAGCACATTGCTGAT | 697 |
Stx2ex probe | FAM-CCCCCAGTTCAGAGTGAGGTCCACG-BHQ1 | 675 |
Stx2f forward | CGCTGTCTGAGGCATCTCC | 608 |
Stx2f reverse | TCCTCTGTACTCTGGAAGAACATTAC | 708 |
Stx2f probe | CFR610-TTATACAATGACGGCTCAGGATGTTGACCTTACC-BHQ2 | 630 |
ompA forwardc | CAGTGATGCGAATCTATGCCAAGATAACACCTGGTACACTGG | 72 |
ompA reversec | TGCCAATCGCATGCTTTGCCTATGTGTGCGTCACCGATGTTG | 517 |
seq ompAf | CAGTGATGCGAATCTATGCCA | NA |
seq ompAr | TGCCAATCGCATGCTTTGCCTA | NA |
Fluorescent probe labels are Q670, Quasar 670; CFO560, CAL Fluor Orange 560; FAM, Carboxyfluorescein and CFR610, CAL Fluor Red 610. Quenchers are BHQ, Black Hole Quencher 1 and 2 (Biosearch, Novato, CA).
Position is relative to the coding region of stx1, stx2A or ompA from EDL933.
ompA forward and reverse sequences in BOLD are homologous to ompA.