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. 2013 Jun 6;8(6):e65716. doi: 10.1371/journal.pone.0065716

Table 6. Incidence of virulence genes in all O157 and non-O157 STEC isolatesa.

Number of O157 STEC isolates (% of total)
Sample source Total recovered stx1 stx2 stx1/stx2b fliC eae hlyA
Domestic ruminantsc 541 200 (37) 522 (96) 181 (33) 541 (100) 54 (100) 541 (100)
Wildlife (feces) 40 22 (55) 40 (100) 22 (55) 40 (100) 40 (100) 40 (100)
Wildlife (swabs) 12 0 (0) 12 (100) 0 (0) 12 (100) 12 (100) 12 (100)
Soil 5 5 (100) 5 (100) 5 (100) 5 (100) 5 (100) 2 (40)
Produce 0 0 (0) 0 (0) 0 (0) 0 (0) 0 (0) 0 (0)
Waterd 60 35 (58) 60 (100) 35 (58) 60 (100) 60 (100) 59 (98)
Sediment 12 8 (67) 12 (100) 8 (67) 12 (100) 12 (100) 12 (100)
Total 670 270 (40) 651 (97) 251 (37) 670 (100) 670 (100) 666 (99)
Number of Non-O157 STEC isolates (% of total)
Sample source Total recovered stx 1 stx 2 stx 1/ stx 2 a sub A eae ehx A
Domestic ruminants 2,590 1,605 (62) 1,944 (75) 962 (37) 754 (29) 577 (23) 2,103 (82)
Wildlife (feces) 304 246 (81) 245 (80) 97 (32) 91 (30) 76 (25) 219 (72)
Wildlife (swabs) 49 13 (26) 47 (96) 5 (10) 5 (10) 26 (53) 18 (37)
Soil 20 5 (25) 19 (95) 4 (20) 3 (15) 5 (25) 8 (40)
Produce 60 25 (41) 47 (77) 12 (20) 34 (56) 5 (8.2) 48 (79)
Water 187 120 (64) 101 (54) 36 (19) 29 (16) 94 (50) 150 (80)
Sediment 74 62 (84) 46 (62) 34 (46) 15 (20) 28 (38) 63 (85)
Total 3,284 2076 (63) 2449 (74) 1150 (35) 931 (28) 811 (25) 2609 (79)
a

Represents isolates saved after screening a larger number of isolates by stx-PCR, O-typing ELISA, MLVA and ompA sequencing. Data from this characterization facilitated selection of isolates to represent those from an individual sample and minimize replication of strains from a sample. STECs isolated by all 3 methods are included.

b

Both stx1 and stx2 present in the same strain.

c

Cattle, alpaca, goats.

d

Isolates from any water sample are included.