Table 2. List of proteins and characteristics of the corresponding antibodies used to detect the proteins.
| Protein | Assay | Antibody | Origin | Dilution | Incubation period |
| NBS1 | WB | rpab | #3002, Cell Signaling Technology | 1∶1000 | Overnight |
| NBS1 | WB | rpab | NB100-143, Novus Biologicals | 1∶10000 | Overnight |
| NBS1 | IP | rpab | NB100-143, Novus Biologicals | 1.0 µl | Overnight |
| mTOR | WB | rpab | #2972, Cell Signaling Technology | 1∶1000 | Overnight |
| mTOR | IP | rpab | #2972, Cell Signaling Technology | 5.0 µl | Overnight |
| Rictor | WB | mmab | H00253260-M01, Abnova | 1∶1000 | Overnight |
| Rictor | IP | mmab | H00253260-M01, Abnova | 5.0 µl | Overnight |
| SIN1 | WB | rpab | A300-910A, Bethyl Laboratories | 1∶2000 | Overnight |
| Flag | WB | mmab | F3165, Sigma-Aldrich | 1∶1000 | 1 hour |
| Flag | IP | mmab | F3165, Sigma-Aldrich | 5.0 µl | Overnight |
| His-tag | WB | mmab | H1029, Sigma-Aldrich | 1∶10000 | Overnight |
| Myc-tag | WB | mmab | LTK Biolaboratories | 1∶500 | Overnight |
| Akt | WB | rpab | #9272, Cell Signaling Technology | 1∶1000 | Overnight |
| p-Akt | WB | rpab | #9271, Cell Signaling Technology | 1∶1000 | Overnight |
| p-GSK-3β | WB | rpab | #9336, Cell Signaling Technology | 1∶1000 | Overnight |
| p-Foxo1/3a | WB | rpab | #9464, Cell Signaling Technology | 1∶1000 | Overnight |
| GSK-3β | WB | rmab | #9315, Cell Signaling Technology | 1∶1000 | Overnight |
| Foxo1 | WB | rpab | #9454, Cell Signaling Technology | 1∶1000 | Overnight |
| Actin | WB | mmab | A5441, Sigma-Aldrich | 1∶20000 | 1 hour |
Abbreviations: WB, Western blot; IP, Immunoprecipitation; mmab, mouse monoclonal antibody; rpab, rabbit polyclonal antibody.