Table 1. Primers used for cDNA synthesis, PCR amplification, and sequencing.
Primer | Sequence | Amino acid motif | Gene | Location |
AN32 | GTYTGCCA | WQT | VP1 | 3009–3002 |
AN33 | GAYTGCCA | WQS | VP1 | 3009–3002 |
AN34 | CCRTCRTA | YDG | VP1 | 3111–3104 |
AN35 | RCTYTGCCA | WQS | VP1 | 3009–3002 |
224 | GCIATGYTIGGIACICAYRT | AMLGTH(I/L/M) | VP3 | 1977–1996 |
222 | CICCIGGIGGIAYRWACAT | M(F/Y)(I/V) | VP1 | 2969–2951 |
AN89 | CCAGCACTGACAGCAGYNGARAYNGG | PALTA(A/V) | VP1 | 2602–2627 |
AN88 | TACTGGACCACCTGGNGGNAYRWACAT | M(F/Y)(I/V)PPG | VP1 | 2977–2951 |
The size of the product was 350 bp to 400 bp.