Figure 1. A combination of three computational algorithms was used to predict small regulatory RNAs in S. pyogenes.

A) The scheme of the computational approach for the prediction of small RNAs in S. pyogenes. The rectangles, ovals, and arrow lines represent computational algorithms, input or output data of computational analyses, and data flow, respectively. The processes were performed to run the algorithms, RNAz, eQRNA and sRNAPredict. B) The candidates predicted by any two algorithms at the same time were considered sRNA candidates. Then, putative cis-regulatory sequences located immediately upstream of annotated ORFs and candidates within prophage sequences were removed from the candidate list. The number of final candidates selected in this manner was 45.