Figure 2. Northern blot identified S. pyogenes sRNAs from the candidates predicted by the computational analysis.

Northern blots were performed with RNA (20 µg) extracted from MGAS315 at the exponential growth phase (Optical density at 600 nm, OD₆₀₀, ∼0.5). The names of the candidate RNA molecules are shown at the bottom of each Northern blot as SSRC (Streptococcal Small RNA Candidate) number. The locations of size markers in nucleotides are shown at the left side of each Northern blot. The approximate sizes of SSRCs calculated based on the location of the size markers are shown in nucleotides (nts) below their names.