Table 2. Primers to be used to create the SSRC21 deletion mutant, ?SSRC21cat.
Name | Sequence# |
5outSSRC21far | GGTATTAAAGGATAGCACATCAAC |
3inSSRC21 | TTTCCCGGGCAATCGACTCATCGCATACAG |
5inSSRC21 | TTTCCCGGGATCTTAGTTAAAATTCAGAATGTATCAG |
3outSSRC21PstI | TTTCTGCAGGGAGGGGAGTTTCCAAAATG |
5outSSRC21 | TTTGGATCCATGTGGTCTATCACAGAAAAAGAAC |
3outSSRC21PstI | TTTCTGCAGGGAGGGGAGTTTCCAAAATG |
5catXmaI | AAACCCGGGGGATTTTTCGCTACGCTCAAATC |
3catXmaI | AAACCCGGGCTTCTTCAACTAACGGGGCAG |
The restriction enzyme sites in primers are indicated in bold.