Table 3.
Methylation Levels within the ALS Pedigrees
| Pedigree | N | Number of Generations with Exp |
Observed Methylation Ratio (HhaI Assay) |
Number of Methylated CpG Sites of Each Pedigree (Bisulfite Sequencing) |
|
|---|---|---|---|---|---|
| Mean ± SD | Mean ± SD | Methylation Level | |||
| Expansion Carriers | |||||
| ped 1 | 4 | 1 | 0.37 ± 0.21 | 1 ± 1.2 | low |
| ped 3 | 2 | 2 | 0.44 ± 0.02 | 2 ± 1.0 | low |
| ped 6 | 4 | 2 | 0.83 ± 0.13 | 13 ± 3.6 | high |
| 1 (43 repeats) | 1 | 0.17 | 0 | no | |
| ped 8 | 2 | 1 | 0.38 ± 0.05 | 4 ± 1.5 | high |
| ped 13 | 1 | 1 | 0.9 | 13 | high |
| ped 16 | 2 | 1 | 1.05 ± 0.00 | 21 ± 4.0 | high |
| ped 17 | 2 | 1 | 0.72 ± 0.02 | 15 ± 7.5 | high |
| Nonexpansion Carriers | |||||
| All pedigrees | 8 | 0.21 ± 0.06 | 0 ± 0.6 | no | |
Abbreviation is as follows: exp, expansion.