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. 2013 Jun 6;92(6):955–964. doi: 10.1016/j.ajhg.2013.04.013

Figure 3.

Figure 3

Colocalization of BICD2 and RAB6A Is Reduced by the SMA-Causing BICD2 Alterations

HeLa cells expressing BICD2-EGFP (green) were stained for RAB6A (red). p.Glu774Gly altered BICD2-EGFP is decreased in RAB6A-positive regions. Also, the p.Ser107Leu altered protein shows reduced colocalization with RAB6A, but not to the same extent as p.Glu774Gly. In addition, the p.Ser107Leu altered BICD2 shows an intense punctual staining in the perinuclear region (arrow). Graphs and error bars represent the mean and SEM. The Pearson’s correlation coefficient (calculated with the ImageJ Coloc2 plugin) is determined in 62, 62, and 61 cells expressing wild-type, p.Ser107Leu, and p.Glu774Gly proteins, respectively, from three independent transfection and immunostaining experiments. ∗p < 0.05, ∗∗∗p < 0.001. The scale bar represents 5 μm.