Figure 5.

p.Ser107Leu Altered BICD2 Leads to Golgi Fragmentation

SH-SY5Y cells stably expressing BICD2-EGFP (green) were immunostained with the Golgi marker Giantin (red). Compared to wild-type and p.Glu774Gly mutant cells, cells with p.Ser107Leu altered BICD2 show a dispersed Golgi apparatus. In addition, in p.Ser107Leu mutant cells, a perinuclear accumulation of BICD2 is apparent (asterisk). Golgi dispersal is represented as a ratio between the minimal rectangle area around the Giantin-positive structures (segmented in ImageJ by automatic intensity thresholding) and the total area of the cell. The graph and error bars represent the mean and SEM of Golgi dispersal measured in 131, 103, and 104 SH-SY5Y cells expressing wild-type, p.Ser107Leu, and p.Glu774Gly proteins, respectively. ^∗∗∗p < 0.001. The scale bar represents 5 μm. The following abbreviation is used: NS, not significant.